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Casey1, 2 Kieran G. A large-scale study of differential gene expression in monocyte-derived macrophages infected with several strains of Mycobacterium avium subspecies paratuberculosis. Current perspectives on Mycobacterium avium subsp. In addition, compared to microarrays, which have a dynamic range constrained by technical factors for example, probe saturation for highly expressed genes, or lack of detectable probe hybridization signal for lowly expressed genesthe dynamic range of RNA-seq is normally limited only by the depth of sequencing used for a particular experimental comparison, thereby leading to higher sensitivity for detection of lowly expressed transcripts. IPA was used to identify the canonical pathways that were enriched for DE genes at both post-infection time points. It is interesting to note that Marfell and colleagues also observed that upregulation of this gene was higher in susceptible animals compared to resistant animals.

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Innate cytokine profiling of bovine alveolar macrophages reveals commonalities and divergence in the response to Mycobacterium bovis and Mycobacterium tuberculosis infection.

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It has also been suggested that inhibition of apoptosis may contribute to the large numbers of infected macrophages that persist in affected tissues 10 Persistence of MAP in macrophages underlies the progression to clinical disease, which is characterized by immunopathology, proliferation of the pathogen, dissemination infection through the host, and ultimately fecal shedding of the pathogen from the host, thus maintaining the cycle of infection 1112 Ribosomal RNA genes were filtered out and lowly expressed genes were also removed with a minimally set threshold of one count per million CPM in at least seven individual libraries the choice of seven libraries is based on the sample size of each treatment group Further analysis, focusing on the uniquely mapping reads demonstrated that a mean of The methods used to isolate, purify, and infect bovine MDM with MAP have been previously described by our group 29 —

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To estimate the dynamic range of the RNA-seq and microarray platforms, the log 2 reads per kilobase per million mapped reads RPKM from the RNA-seq data and the log 2 intensities from the microarray data were analyzed as described by Nalpas et al. A comparison of massively parallel nucleotide sequencing with oligonucleotide microarrays for global transcription profiling. The relatively low overlap of DE genes between the two time points most likely represents evolution of the MDM transcriptional response to MAP infection over the time course. Biochim Biophys Acta The role of IL in immune regulation during M.